NCI-H1048-CDK2-HiBiT-KI-2A2 Cell Line

Cyclin-dependent kinases (CDKs) are key players in cell cycle regulation. Cyclin-dependent kinase 2 (CDK2) is best known for its key role during cell cycle progression. This cyclin-dependent kinase (CDK) family member is involved in DNA synthesis, G1/S phase transition, and G2 progression modulation. While the monomeric form of CDK2 is inactive, the kinase becomes active, like many other CDKs, when it forms a functional heterodimeric complex with one of its two regulatory partners – Cyclins A and E. CDK2 modulates a variety of oncogenic signaling pathways because it governs the phosphorylation of a wide range of transcription factors: SMAD3, FOXM1, FOXO1, ID2, UBF, NFY, B-MYB, and MYC. Unsurprisingly, the aberrant activation of CDK2, which occurs in many human cancers, leads to uncontrolled cell proliferation during oncogenesis. Likewise, oncogenic processes of several cancers are also associated with high levels of the two regulatory subunits of CDK2. Altogether, both the activity of CDK2 and its regulatory subunits appear to be important components of oncogenesis.