Cell Proliferation Assay

Cell Proliferation Assay Overview

Cell viability and cell proliferation assays are essential components of any tumor inhibitor development program. Cell proliferation assays are widely employed for the initial assessment of the therapeutic effects of antineoplastic agents. The most commonly used method for cell proliferation assays is the 2D cell proliferation assay. In this assay, tumor cells are cultured either in suspension in a culture medium or as monolayers in cell culture plates. After treatment with compounds, cell viability is measured using the CTG reagent at the end of the experiment. Due to the simplicity of the experimental procedure, this standardized assay allows for the rapid testing of a large number of compounds simultaneously, and the 3D cell proliferation assay can also be integrated into this standard procedure.

We possess the largest BaF3 engineered cell bank globally, enabling us to conduct rapid, high-throughput screening of compounds across a wide panel of cell lines. In our standard experiments, the drug incubation period extends up to 3 days, which can be extended to a maximum of 14 days if required by the project. Additionally, 3D experiments are always a valuable option for studying organoids.

Figure: 2D Cell Proliferation Assay

Figure: 3D Cell Proliferation Assay

All in vitro Services

The 2D Cell Proliferation Assay, a primary method for cell growth studies, focuses on tumor cells cultured in suspension or monolayers. It allows for comprehensive analysis, highlighting cell development dynamics.


The value of tumor cell lines, as research models and drug discovery tools, is greatly enhanced when there is an understanding of the underlying genetic abnormalities that drive their phenotype. Kyinno provides customized cell panel studies to facilitate your drug discovery and biomarker programs.

Organoids, sophisticated 3D organ replicas grown in vitro, are vital in biomedical research, aiding disease modeling, drug testing, and understanding organ development. KYinno excels in cultivating organoids from diverse tumor tissues, collaborating closely with cancer hospitals to ensure translational relevance. 

Internalization Assay evaluates cellular absorption rates of monoclonal antibodies, vital for advancing anti-cancer and anti-inflammatory therapies. This process is essential for developing anti-cancer and anti-inflammatory treatments and refining oncology and inflammation treatments.
ELISA is an indispensable technique that stands out for its high sensitivity and specificity in antigen-antibody binding interactions. Its precision ensures it remains the preferred choice for many immune response studies.

FACS Binding Assay or Fluorescence-Activated Cell Sorting, is a pivotal cellular biology research technique for binding evaluation. It offers insights into cellular binding mechanisms, enriching our understanding of cell interactions.

Immuno-Oncology Assay is a core tool in tumor immunology, providing detailed information designed to analyze interactions between tumors and immune responses.This deep understanding accelerates advancements in tumor immunology research.
ADCC Assay measures antibody efficacy in biological research. A standard method for understanding antibody performance. It rigorously tests their functionality and efficacy, providing insights into antibody-mediated cell destruction processes.