Customized Cell Line Construction

Fast Turnaround Custom Cell Line Development

Creating stable cell lines can be challenging and time-intensive. Kyinno Bio provides user-friendly Custom Cell Line Development services that can assist you in streamlining your research and accelerating your project timelines.

Cell line engineering is essential for developing tools for protein expression, pathway studies, inhibitor/activator studies, positive and negative controls for immunoassays, CAR-T research, dosage response screening, and many other applications. Explore how Kyinno Bio’s advanced solutions can elevate your research. Contact us today to get started!

Service Highlights

  1. you will receive multiple clones
  2. fast turnaround time: 8 weeks
  3. many parental cell lines to choose from
  4. robust QC, guaranteed to meet specifications: expression levels, functional activity, robust outputs, etc.
  5. PhD-level support and consultation every step of the way

Available Custom Cell Line Formats

Kyinno Bio has the largest pre-made engineered cell bank in the world, with over 4000 cell lines ready to buy. However if you do not find the cell line that fulfills your requirements, we can custom make them for you. There are 6 common cell line formats that we can develop:

  1. Overexpression: positive controls for in vitro and in vivo assays. Study how modulation of a protein affects mechanism of action
  2. Reporter: in vitro high throughput screening.
  3. Knockout/Knockin: negative controls for in vitro assays. Study how knockout of a protein affects mechanism of action
  4. Co-stimulatory: reporter assay co-culture. Immunotherapy Research
  5. CAR-T: Immunotherapy Development
  6. Bioluminescent: signal cell in xenograft models.

Available Promotors and Reporters

Promotors

Reporters

Our processes for custom cell line construction

Our custom cell line development service follows a well defined series of steps. Each step is critical to ensure the quality and reliability of the cell lines. Here’s a detailed breakdown of our workflow:

  1. Introduction of Vectors: Introduce lentivirus or plasmids into the target cells.
  2. Obtaining Polyclonal Pool: Use antibiotic selection or fluorescence sorting to obtain a polyclonal pool.
  3. Seeding into Plates: Seed the polyclonal pool into 96-well plates to isolate single clones.
  4. Verification of Clones: Verify the single clones to confirm the target cell line.
  5. Quality Testing: Perform quality testing on each cell line to ensure absence of pathogen contamination and accuracy of STR sequencing.
flowchart of stable overexpression cell line construction
flowchart of stable gene-editing cell line construction

Build Your Own Cell Line