Isotype controls are a type of primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in in vitro and in vivo studies. Isotype controls are often used to measure the level of non-specific background signal caused by binding non-specifically to Fc receptors on the cell surface, or other cellular molecules.
Chinese hamster ovary (CHO) host cell line is the most popular mammalian system of therapeutic proteins production. The isotype controls produced by CHO line share similar posttranslational modifications with the therapeutic proteins, especially in N‐linked glycosylation, which are useful benchmark in the discovery and development of therapeutic antibodies.
Specifications
Catalog Number:
KP-2023
Antibodies Name:
Mouse-IgG2a-isotype-kappa
Host Cell Line:
EXPI-CHO
Target:
HEL
Species Reactivity:
Chicken
Application:
ELISA
Purification Method:
Affinity purified
Concentration:
>2mg/mL
Purity:
>95% by SDS-PAGE and SEC-HPLC
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Sterility:
0.2μm filtered
Formulation:
20mM sodium citrate,150mM NaCl, pH5.5
Storage:
Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.
Bio Safety Level:
1
Validation:
SDS-PAGE | SEC-HPLC | ELISA
Characterization
Application
References
1.Vidarsson, G., Dekkers, G. & Rispens, T. IgG Subclasses and Allotypes: From Structure to Effector Functions. Front. Immunol. 5, (2014).
2.Zhong, X. et al. Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins. Biotechnol. Prog. 35, e2724 (2019).
