HEK293T Rat TF Cell Line

Catalog Number:

KC-2573

Cell Line Name:

HEK293T Rat TF Cell Line

Host Cell Line:

Human HEK293T cell line

Description:

Quantity:

One vial of frozen cells (5×10⁶ per vial)

Stability:

Stable in culture over a minimum of 10 passages

Application:

Drug screening and biological assays

Freeze Medium:

70% DMEM+20% FBS+10% DMSO

Propagation Medium:

DMEM+10%FBS+1μg/mL Puromycin

Selection Marker:

Puromycin

Morphology:

Epithelial

Subculture:

Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 10⁵ cells/mL

Incubation:

37 °C with 5% CO₂

Storage:

Liquid nitrogen immediately upon receiving

Doubling Time:

Approximately 30 hours

Mycoplasma Status:

Negative