KB-1034

7D4-mIgG2a

Background

CD25, in association with CD122 and CD132, forms the high-affinity cell-surface receptor for the IL-2 cytokine (IL-2R). The anti-mCD25 7D4 mAb does not block IL-2 binding to CD25 and thus preserves IL-2 signaling. This particular aspect has been highlighted as an important parameter to achieve strong antitumor responses in mouse models.

Specifications

Catalog Number:
KB-1034
Cell Line Name:
7D4-mIgG2a
Price:
0
Host Cell Line:
EXPI-CHO
Target:
Mouse-CD25
Species Reactivity:
Mouse
Application:
ELISA
Purification Method:
Affinity purified
Concentration:
>2mg/mL
Purity:
>95% by SDS-PAGE and SEC-HPLC
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Sterility:
0.2μm filtered
Formulation:
20mM sodium citrate,150mM NaCl, pH5.5
Storage:
Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.
Bio Safety Level:
1
Validation:
SDS-PAGE | SEC-HPLC | ELISA

References

1.Williams MA, Tyznik AJ, and Bevan MJ 2006. Interleukin-2 signals during priming are required for secondary expansion of CD8+ memory T cells. Nature 441: 890–893. 2.Laurence A, Tato CM, Davidson TS, Kanno Y, Chen Z, Yao Z, Blank RB, Meylan F, Siegel R, Hennighausen L, Shevach EM, and O’Shea J J 2007. Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation. Immunity 26: 371–381.
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