KC-0152

U2OS-REV-GFP-Cell-Line

19933

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Background of U2OS-REV-GFP-Cell-Line

Catalog Number	KC-0152
Cell Line Name	U2OS-REV-GFP-Cell-Line
Host Cell Line	Human U2OS cell line
Description	Stable U2OS clone expressing exogenous human GFP tagged REV
Quantity	One vial of frozen cells (≥2×10⁶ cells)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% McCoy's 5a+20%FBS+10%DMSO
Propagation Medium	McCoy's 5a+10%FBS+2µg/mL Puromycin
Selection Marker	Puromycin
Subculture	Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Mycoplasma Status	Negative
In Vivo Validation	NA

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.