KC-2599-DW

RM-1-Trop2 Cell Line

60541

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Background of RM-1-Trop2 Cell Line

TACSTD2 encodes trophoblast cell-surface antigen 2 (Trop2), a type I transmembrane glycoprotein involved in epithelial cell signaling, adhesion and proliferation. Trop2 is frequently expressed in solid tumors and is widely studied as a tumor-associated surface antigen and therapeutic target.

Specifications

Catalog Number	KC-2599-DW
Cell Line Name	RM-1-Trop2 Cell Line
NCBI/UniProt Accession Number	NM_002353.3
Clone Number	4#
Host Cell Line	RM-1
Description	Stable RM-1 cell line expressing exogenous human Trop2
Quantity	One vial of frozen cells (≥2×10⁶ cells)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% RPMI1640+20% FBS+10% DMSO
Propagation Medium	RPMI1640+10%FBS+1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial-like
Subculture	Split at a ratio of 1:2–1:3 every 2–3 days
Incubation	37°C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Estimated 30 hours
Mycoplasma Status	Negative
In Vivo Validation	YES

Cell Line Generation

The RM-1-Trop2 cell line was generated using a lentiviral vector expressing the human Trop2 sequence.

Characterization

Figure 1: Characterization of human TROP2 overexpression in the RM-1-Trop2 stable clone using FACS.

Cell Resuscitation

Pre-warm complete culture medium (RPMI1640 and 10% FBS) in a 37°C water bath.
Rapidly thaw the cryovial in a 37°C water bath for 1–2 minutes with gentle agitation.
Transfer the vial to a biosafety cabinet and disinfect the exterior with 70% ethanol.
Aseptically transfer the cell suspension dropwise into a sterile centrifuge tube containing 9.0 mL of pre-warmed complete medium.
Centrifuge at approximately 125×g for 5–7 minutes at room temperature; carefully aspirate the supernatant without disturbing the cell pellet.
Gently resuspend the pellet in an appropriate volume of complete medium and transfer the suspension into a T25 flask.
Incubate the flask at 37°C in a humidified 5% CO₂ incubator.
Assess cell viability and morphology after 24 hours. If cells appear healthy, replace the medium with fresh medium supplemented with 1 μg/mL puromycin.
Subculture the cells at a ratio of 1:2–1:3 every 2–3 days upon reaching 80%–90% confluency.

Cell Freezing

Prepare the freezing medium (70% RPMI1640, 20% FBS and 10% DMSO) freshly before use.
Pre-chill the freezing medium on ice and label the cryovials accordingly.
Transfer the cell suspension to a sterile conical tube and perform a cell count to determine total viability and density.
Centrifuge the cells at 250×g for 5 minutes at room temperature; carefully aspirate the supernatant.
Gently resuspend the cell pellet in chilled freezing medium, ensuring a minimum cell density of 3×10⁶ cells/mL.
Aliquot 1 mL of the cell suspension into each pre-labeled cryovial.
Place the cryovials into a CoolCell® container and store at -80°C overnight for controlled-rate cooling.
Transfer the cryovials to liquid nitrogen for long-term storage the following day.

References

Shen M, Liu S, Stoyanova T. The role of Trop2 in prostate cancer: an oncogene, biomarker, and therapeutic target. American Journal of Clinical and Experimental Urology. 2021;9(1):73–87.
Wen Y, Ouyang D, Zou Q, et al. A literature review of the promising future of TROP2: a potential drug therapy target. Annals of Translational Medicine. 2022;10(24):1403.
Sakach E, Sacks R, Kalinsky K. Trop-2 as a Therapeutic Target in Breast Cancer. Cancers. 2022;14(23):5936.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.