KC-2397

RM1-FOLH-Luc2 Cell Line

Home » RM1-FOLH-Luc2 Cell Line

Background

FOLH, also known as PSMA, is a type II transmembrane glycoprotein belonging to the M28 peptidase family, which is a glutamate-preferrng carboxy-peptidase. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelia cellsfrom breast, duodenum, and kidney tissues, and in prostate cancer. In the prostate the protein is up-requlated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Specifications

Catalog Number:
KC-2397
Cell Line Name:
RM1 FOLH Luc2 Cell Line
Host Cell Line:
Mouse RM1 cell line
Description:
Quantity:
One vial of frozen cells (5X106 per vial)
Stability:
Stable in culture over a minimum of 10 passages
Application:
Drug screening and biological assays
Freeze Medium:
70% RPMI1640+20% FBS+10% DMSO
Propagation Medium:
RPMI1640+10%FBS+2μg/mL Puromycin
Selection Marker:
Puromycin
Morphology:
Fibroblast-like
Subculture:
Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation:
37 °C with 5% CO2
Storage:
Liquid nitrogen immediately upon receiving
Doubling Time:
Approximately 30 hours
Mycoplasma Status:
Negative

Cell Line Generation

RM1 Human FOLH Luc2 cell line was generated using a lentiviral vector expressing the human FOLH sequence.

Characterization

0

Application

0

Cell Resuscitation

1. Prewarm culture medium (RPMI1640 + 10% FBS + 2μg/mL Puromycin) in a 37°C water bath. 2. Thaw the frozen vial in a 37°C water bath for 1-2 minutes. 3. Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol. 4. Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium. 5. Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet. 6. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask. 7. Incubate the flask at 37°C, 5% CO2 incubator. 8. Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL.

Cell Freezing

1. Prepare the freezing medium (70% RPMI-1640 + 20% FBS + 10% DMSO) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3×106 cells/mL in chilled freezing medium. 6. Aliquot 1 mL of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage

References

1. Prostate-Specific Membrane Antigen (PSMA) Theranostics for Treatment of Oligometastatic Prostate Cancer.PMID: 34829977 PMCID: PMC8621856 DOI: 10.3390/ijms222212095 2. GCPII imaging and cancer. C A Foss 1, R C Mease, S Y Cho, H J Kim, M G Pomper.PMID: 22304713 PMCID: PMC4076792 DOI: 10.2174/092986712799462612 3. Is prostate-specific membrane antigen a multifunctional protein Ayyappan? K Rajasekaran 1, Gopalakrishnapillai Anilkumar, Jason J Christiansen.PMID: 15840561 DOI: 10.1152/ajpcell.00506.2004
Please enable JavaScript in your browser to complete this form.