KC-3360
HLA-A encodes major histocompatibility complex, class I, A and is also known as HLAA. HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons.
| Catalog Number | KC-3360 |
|---|---|
| Cell Line Name | PC9-HLA-A0101 Cell Line |
| NCBI/UniProt Accession Number | NM_001242758.1 |
| Clone Number | 2# |
| Host Cell Line | PC9 |
| Description | PC9-HLA-A0101 is a stable engineered cell line expressing human HLA-A0101 |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% RPMI1640+20% FBS+10% DMSO |
| Propagation Medium | RPMI1640+10%FBS+300μg/mL Hygromycin B |
| Selection Marker | Hygromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1: Characterization of HLA-A overexpression in the PC9-HLA-A0101 stable clone using FACS.
