KC-2181
Fibroblast activation protein alpha (FAP; important alias: FAPalpha) is a type II transmembrane serine protease of the dipeptidyl peptidase 4 family. FAP is selectively expressed in reactive stromal fibroblasts and cancer-associated fibroblasts of epithelial tumors, while its expression is very low in most normal adult tissues. FAP promotes tumor growth, invasion, metastasis and immunosuppression through extracellular matrix remodeling, intracellular signaling, angiogenesis and epithelial-to-mesenchymal transition, and has also been linked to fibrosis and other inflammatory conditions. The abundant and stable expression of FAP in the tumor stroma makes it an attractive target for cancer diagnostics and therapeutics, including FAP-targeted radioligand imaging and therapy, antibody-drug conjugates and CAR-T cells under active development.
| Catalog Number | KC-2181 |
|---|---|
| Cell Line Name | NIH/3T3-mouse-FAP Cell Line |
| NCBI/UniProt Accession Number | NM_007986.3 |
| Host Cell Line | NIH/3T3 |
| Description | NIH/3T3 cell line stably expressing exogenous mouse FAP gene |
| Quantity | One vial of frozen cells (≥2×106 cells) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10%FBS+1μg/mL Puromycin |
| Selection Marker | Puromycin |
| Morphology | Fibroblast-like |
| Subculture | Split at a ratio of 1:4–1:5 every 2–3 days |
| Incubation | 37°C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 30 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1: Characterization of mouse FAP overexpression in the NIH/3T3-mouse-FAP stable clone using FACS.
Figure 2: Characterization of the mouse FAP sequence in the NIH/3T3-mouse-FAP stable clone using Sanger sequencing.
