KC-5743
KLK2 encodes kallikrein related peptidase 2 and is also known as hK2. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants.
| Catalog Number | KC-5743 |
|---|---|
| Cell Line Name | 293T-mem-cyno-KLK2 Cell Line |
| NCBI/UniProt Accession Number | A0A2K5VYN9 |
| Clone Number | 9# |
| Host Cell Line | 293T |
| Description | 293T-mem-cyno-KLK2 is a stable engineered cell line expressing cyno mem-cyno-KLK2 |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10%FBS+1μg/mL puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1: Characterization of mem cyno KLK2 overexpression in the 293T-mem-cyno-KLK2 stable clone using FACS.
Figure 2: Characterization of mem cyno KLK2 in the 293T stable clone using PCR sequencing.
