KC-1793

CHO-K1 5HTR1F CRE-Luc2 Reporter Cell Line

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Background

Specifications

Catalog Number:
KC-1793
Cell Line Name:
CHO-K1 5HTR1F CRE-Luc2 Reporter Cell Line
Host Cell Line:
Chinese hamster ovary CHO-K1 cell line
Description:
Stable CHO-K1 clone expressing exogenous human 5HTR1F and luciferase under the control of CRE response element
Quantity:
One vial of frozen cells (5×106 per vial)
Stability:
Stable in culture over a minimum of 10 passages
Application:
Drug screening and biological assays
Freeze Medium:
70% RPMI1640+20% FBS+10% DMSO
Propagation Medium:
RPMI1640+10%FBS+10µg/mL Puromycin+750µg/mL Hygromycin B
Selection Marker:
Puromycin
Morphology:
Epithelial-like
Subculture:
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 105 cells/mL
Incubation:
37 °C with 5% CO2
Storage:
Liquid nitrogen immediately upon receiving
Doubling Time:
Mycoplasma Status:
Negative

Cell Line Generation

Characterization

Application

Cell Resuscitation

Cell Freezing

References

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