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KC-2723

Ba/F3-BCR-ABL-P465S Cell Line

Home » Ba/F3-BCR-ABL-P465S Cell Line

Background

Specifications

Catalog Number:
KC-2723
Cell Line Name:
Ba/F3-BCR-ABL-P465S Cell Line
Host Cell Line:
Mouse Ba/F3 cell line
Description:
Stable Ba/F3 clone expressing exogenous BCR-ABL gene bearing P465S point mutation in ABL part
Quantity:
One vial of frozen cells (5×106 per vial)
Stability:
Stable in culture over a minimum of 10 passages
Application:
Drug screening and biological assays
Freeze Medium:
70% RPMI1640+20% FBS+10% DMSO
Propagation Medium:
RPMI1640+10%FBS
Selection Marker:
Puromycin
Morphology:
Mostly single, round (some polymorph) cells in suspension
Subculture:
Split saturated culture 1:10 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation:
37 °C with 5% CO2
Storage:
Liquid nitrogen immediately upon receiving
Doubling Time:
Approximately 20 hours
Mycoplasma Status:
Negative

Cell Line Generation

Characterization

Application

Cell Resuscitation

Cell Freezing

References

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