KC-1854
GPRC5B encodes G protein-coupled receptor class C group 5 member B and is also known as MLC3. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants.
| Catalog Number | KC-1854 |
|---|---|
| Cell Line Name | 293T-GPRC5B Cell Line |
| NCBI/UniProt Accession Number | NM_016235.3 |
| Host Cell Line | 293T |
| Description | 293T-GPRC5B is a stable engineered cell line expressing human GPRC5B |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10%FBS+1μg/mL Puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1:Characterization of 293T-GPRC5B cell line stable clone using FACS.
Figure 2: Characterization of 293T-GPRC5B cell line stable clone using PCR sequencing.
