KC-2554
CLDN18 encodes claudin 18 and is also known as SFTA5. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.
| Catalog Number | KC-2554 |
|---|---|
| Cell Line Name | 293T-cyno-CLDN18.2 Cell Line |
| NCBI/UniProt Accession Number | NM_001002026 |
| Host Cell Line | 293T |
| Description | 293T-cyno-CLDN18.2 is a stable engineered cell line expressing cyno CLDN18.2 |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10%FBS+1μg/mL Puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1: Characterization of cyno-CLDN18.2 overexpression in the 293T-cyno-CLDN18.2 stable clone using FACS.
