KC-3747
Cadherin 3 (CDH3), also known as P-cadherin, is a calcium-dependent transmembrane cell-cell adhesion glycoprotein of the classical cadherin family. It is expressed in placental, basal epithelial and myoepithelial tissues, where it mediates adherens junctions, cell sorting and tissue architecture. CDH3/P-cadherin is upregulated in a subset of breast, lung, gastric, colorectal and other carcinomas, where its expression correlates with cancer stem-cell features, epithelial plasticity, invasion and adverse prognosis. As a cell-surface antigen with restricted normal-tissue distribution, CDH3 is being developed as a target for antibody-drug conjugates, T-cell-engaging bispecific antibodies and other precision cancer therapies, including conjugates designed to deliver cytotoxic payloads to CDH3-positive tumor cells.
| Catalog Number | KC-3747 |
|---|---|
| Cell Line Name | 293T-cyno-CDH3 Cell Line |
| NCBI/UniProt Accession Number | XM_005592357.5 |
| Clone Number | 3# |
| Host Cell Line | 293T |
| Description | The 293T-cyno-CDH3 cell line stably overexpresses cyno CDH3 |
| Quantity | One vial of frozen cells (≥2×106 cells) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20%FBS+10%DMSO |
| Propagation Medium | DMEM+10%FBS+1µg/mL Puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial-like |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37°C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 30 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1: Characterization of cyno CDH3 overexpression in the 293T-cyno-CDH3 stable clone using FACS.
Figure 2: Characterization of the cyno CDH3 sequence in the 293T-cyno-CDH3 stable clone using Sanger sequencing.
