KC-5490
ERBB2 encodes erb-b2 receptor tyrosine kinase 2 and is also known as NEU. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
| Catalog Number | KC-5490 |
|---|---|
| Cell Line Name | U87MG-ERBB2-Luc2 Cell Line |
| Clone Number | 8# |
| Host Cell Line | U87MG-Luc2 |
| Description | U87MG-ERBB2-Luc2 is a stable engineered cell line expressing human ERBB2-Luc2 |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% (MEM+20% FBS+10% DMSO |
| Propagation Medium | (MEM+0.01mM NEAA)+10%FBS+1%Sodium pyruvate+2μg/mL puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1: Characterization of ERBB2 overexpressing in U87MG-Luc2-ERBB2 stable clones using FACS.
Figure 2. Characterization of U87MG-Luc2-ERBB2 cell line stable clone using Bright-Glo Detection System in the conditions of different cell number.
Figure3: Characterization of U87MG-Luc2-ERBB2 cell line stable clone using PCR sequencing.
