KC-5650

SK-OV-3-ST6GALNAC1 Cell Line

59991

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Background of SK-OV-3-ST6GALNAC1 Cell Line

ST6GALNAC1 encodes ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 and is also known as STYI. Glycosylation of proteins affects cell-cell interaction, interactions with the matrix, and the functions of intracellular molecules. ST6GALNAC1 transfers a sialic acid, N-acetylneuraminic acid (NeuAc), in an alpha-2,6 linkage to O-linked GalNAc residues. The cancer-associated sialyl-Tn (sTn) antigen is formed by ST6GALNAC1-catalyzed sialylation of GalNAc residues on mucins (Ikehara et al., 1999 [PubMed 10536037]; Sewell et al., 2006 [PubMed 16319059]).[supplied by OMIM, Mar 2008]

Specifications

Catalog Number	KC-5650
Cell Line Name	SK-OV-3-ST6GALNAC1 Cell Line
NCBI/UniProt Accession Number	NM_018414.5
Clone Number	1#
Host Cell Line	SK-OV-3
Description	SK-OV-3-ST6GALNAC1 is a stable engineered cell line expressing human ST6GALNAC1
Quantity	One vial of frozen cells (≥2×10⁶ cells/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% McCoy's 5a+20% FBS+10% DMSO
Propagation Medium	McCoy's 5a+10% FBS+1μg/mL puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split at a ratio of 1:4–1:6 every 2–3 days
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Estimated 19–36 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

SK-OV-3-ST6GALNAC1 cell line was generated using a lentiviral vector expressing the human ST6GALNAC1 sequence.

Characterization

Figure 1: Characterization of human ST6GALNAC1 overexpression in the SKOV3 human ST6GALNAC1 stable clone using PCR sequence

Figure 2: Characterization of human ST6GALNAC1 overexpression in the SKOV3 human ST6GALNAC1 stable clone using FACS.

Cell Resuscitation

Pre-warm complete culture medium (basal medium and 10% FBS) in a 37°C water bath.
Rapidly thaw the cryovial in a 37°C water bath for 1-2 minutes with gentle agitation.
Transfer the vial to a biosafety cabinet, and disinfect the exterior with 70% ethanol.
Aseptically transfer the cell suspension dropwise into a sterile centrifuge tube containing 9.0 mL of pre-warmed complete medium.
Centrifuge at approximately 125 × g for 5–7 minutes at room temperature, carefully aspirate the supernatant without disturbing the cell pellet.
Gently resuspend the pellet in an appropriate volume of complete medium and transfer the suspension into a T25 flask.
Incubate the flask in a 37°C in a humidified 5% CO₂ incubator.
Assess cell viability and morphology after 24 hours. If cells appear healthy, replace the medium with fresh medium supplemented with the appropriate selective antibiotic.
Subculture the cells at a ratio of 1:4–1:6 every 2–3 days upon reaching 80%–90% confluency.

Cell Freezing

Prepare the freezing medium (70% basal medium, 20% FBS and 10% DMSO) freshly before use.
Pre-chill the freezing medium on ice and label the cryovials accordingly.
Transfer the cell suspension to a sterile conical tube and perform a cell count to determine total viability and density.
Centrifuge the cells at 250×g for 5 minutes at room temperature; carefully aspirate the supernatant.
Gently resuspend the pellet in chilled freezing medium, ensuring a minimum cell density of 3×10⁶ cells/mL.
Aliquot 1 mL of the cell suspension into each pre-labeled cryovial.
Place the cryovials into a CoolCell® container and store at -80°C overnight for controlled-rate cooling.
Transfer the cryovials to the liquid nitrogen for long-term storage the following day.

References

Umino R, Ishimoto-Namiki U, Naito C et al. Novel insights into immunohistochemical analysis of intrahepatic cholangiocarcinoma: ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 (ST6GalNAc1) as a new marker for large duct type. Virchows Archiv : an international journal of pathology. 2026 Aug 14;:. PMID: 42595919. doi: 10.1007/s00428-026-04675-z.
Sodini A, Casali E, Travecedo MA et al. Studies on a Sulfoxide-Bridged Tn Antigen Mimetic: Interaction with Macrophage Galactose Lectin and Inhibition of Sialyltransferase ST6GALNAC1. ACS omega. 2026 Apr 21;11(15):23350-23358. PMID: 42040468. doi: 10.1021/acsomega.6c00385.
Fu T, Wang Y, Wang L et al. ST6GALNAC1 mediates sialylation of mucins in non-small cell lung cancer to evade immune surveillance. Journal of thoracic disease. 2025 Aug 31;17(8):6076-6089. PMID: 40950850. doi: 10.21037/jtd-2025-660.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.