KC-5540
TSHR encodes thyroid stimulating hormone receptor and is also known as LGR3. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.
| Catalog Number | KC-5540 |
|---|---|
| Cell Line Name | 293T-cyno-TSHR-D240 Cell Line |
| NCBI/UniProt Accession Number | A0A2K5X226 |
| Clone Number | 11# |
| Host Cell Line | 293T |
| Description | 293T-cyno-TSHR-D240 is a stable engineered cell line expressing cyno TSHR-D240 |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10%FBS+1μg/mL puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. Characterization of cyno-TSHR-D240 over-expression in the 293T-cyno-TSHR-D240 stable clone using FACS.
Figure 2. Characterization of 293T-cyno-TSHR-D240 cell line stable clone using PCR sequencing.
