KC-3495
Stable HEK293T cell line expressing exogenous human ACVR2B gene
ACVR2B encodes activin A receptor type 2B and is also known as HTX4. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors.
| Catalog Number | KC-3495 |
|---|---|
| Cell Line Name | 293T-ACVR2B Cell Line |
| NCBI/UniProt Accession Number | NM_001106 |
| Clone Number | 2# |
| Host Cell Line | 293T |
| Description | 293T-ACVR2B is a stable engineered cell line expressing human ACVR2B |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10%FBS+1μg/mL Puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. Characterization of ACVR2B over-expression in the 293T-ACVR2B stable clone using qPCR.
Figure 2. Characterization of ACVR2B over-expression in the 293T-ACVR2B stable clone using PCR.
