KC-3003
DDR1 encodes discoidin domain receptor tyrosine kinase 1 and is also known as CAK. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
| Catalog Number | KC-3003 |
|---|---|
| Cell Line Name | Ba/F3-DDR1 Cell Line |
| NCBI/UniProt Accession Number | NM_013993.3 |
| Clone Number | 4# |
| Host Cell Line | Ba/F3 |
| Description | Ba/F3-DDR1 is a stable engineered cell line expressing human DDR1 |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% RPMI1640+20% FBS+10% DMSO |
| Propagation Medium | RPMI1640+10%FBS+1μg/mL Puromycin+8ng/mL mouse IL-3 |
| Selection Marker | Puromycin |
| Morphology | Mostly single, round (some polymorph) cells in suspension |
| Subculture | Split at a ratio of 1:10 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 14–22 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure1: Characterization of DDR1 overexpression in Ba/F3-DDR1 stable clone using FACS.
Figure2: Characterization of DDR1 overexpressing in Ba/F3-DDR1 stable clone using PCR sequencing.
