KC-3760
AQP4 encodes aquaporin 4 and is also known as MIWC. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon.
| Catalog Number | KC-3760 |
|---|---|
| Cell Line Name | 293T-AQP4-M23 Cell Line |
| Host Cell Line | 293T |
| Description | 293T-AQP4-M23 is a stable engineered cell line expressing human AQP4-M23 |
| Quantity | One vial of frozen cells (≥2×10⁶ cells/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM + 20% FBS + 10% DMSO |
| Propagation Medium | DMEM + 10% FBS + 1μg/mL Puromycin |
| Selection Marker | Puromycin |
| Morphology | Epithelial |
| Subculture | Split at a ratio of 1:4–1:6 every 2–3 days |
| Incubation | 37 °C with 5% CO₂ |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Estimated 19–36 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure: Characterization of AQP4-M23 overexpression in 293T stable clone using QPCR.
