KB-1200

REGN7075-EGFR-hIgG1

Background

The ErbB receptor tyrosine kinase family consists of four members, ErbB1 (epidermal growth factor receptor, EGFR), ErbB2, ErbB3, and ErbB4, which are expressed on a plethora of cell types, including epithelial, endothelial , neuronal and glial, bone, adipose, liver, and cardiovascular cells. Following ligand binding and activation, oligomerisation of ErbB family members occurs. ErbB signalling is then induced through phosphorylation of intracellular domains, resulting in the activation of several major intracellular signalling pathways, including mitogen activated protein (MAP) kinase, nuclear factor kappa B (NF-ĸB), phosphoinositide 3 (Pi3) kinase, and janus kinase/signal transducer and activator of transcription (JAK/STAT) pathways. ErbB-associated signalling pathways govern a wide variety of outcomes, including cell survival, proliferation, cell death/apoptosis, angiogenesis, adhesion, differentiation, and migration/invasion.

Specifications

Catalog Number:
KB-1200
Cell Line Name:
REGN7075-EGFR-hIgG1
Price:
0
Host Cell Line:
EXPI-CHO
Target:
EGFR
Species Reactivity:
Human
Application:
ELISA
Purification Method:
Affinity purified
Concentration:
>2mg/mL
Purity:
>95% by SDS-PAGE and SEC-HPLC
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Sterility:
0.2μm filtered
Formulation:
20mM sodium citrate,150mM NaCl, pH5.5
Storage:
Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.
Bio Safety Level:
1
Validation:
SDS-PAGE | SEC-HPLC | ELISA

References

1.Tao R.-H., Maruyama I.N. All EGF(ErbB) receptors have preformed homo- and heterodimeric structures in living cells. J. Cell Sci. 2008;121:3207–3217.  2.Hoffmann I. Activation of ErbB2 receptor tyrosine kinase supports invasion of endothelial cells by Neisseria meningitidis. J. Cell Biol. 2001;155:133–144.  3.Torp S.H. Epidermal growth factor receptor expression in human gliomas. Cancer Immunol. Immunother. 1991;33:61–64.  4.Oda Y. Expression of growth factors and their receptors in human osteosarcomas. Immunohistochemical detection of epidermal growth factor, platelet-derived growth factor and their receptors: its correlation with proliferating activities and p53 expression. Gen. Diagn. Pathol. 1995;141:97–103.  5.Donnenberg A.D. The cell-surface proteome of cultured adipose stromal cells. Cytometry. 2015;87:665–674.  6.Scheving L.A. Loss of hepatocyte ERBB3 but not EGFR impairs hepatocarcinogenesis. Am. J. Physiol. Gastrointest. Liver Physiol. 2015;309:G942–G954.  7.Brea M.S. Epidermal growth factor receptor silencing blunts the slow force response to myocardial stretch. J. Am. Heart Assoc. 2016;5:e004017.
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