KB-1126

JNJ372-Met-hIgG1

Background

JNJ372-Met-hlgG1, a high affinity-specific humanized IgG1 antibody against HGFR. HGFR also known as the only high affinity receptor for hepatocyte growth factor,plays vital physiological functions during embryogenesis, angiogenesis, and repair processes.Under malignant conditions, the interaction between HGF and cMET triggers an intricate system of downstream mitogen-activated protein kinase (MAPK)/ERK and PI3K/AKT signaling cascades, thus resulting in uncontrolled cell proliferation, epithelial‐ mesenchymal transition (EMT) and metastatic spread. Antibodies to HGFR are used in humans as targeted therapies for a variety of cancers that included patients with bladder cancer,hepatocellular cancer and nonsmall cell lung cancer.

Specifications

Catalog Number:
KB-1126
Cell Line Name:
JNJ372-Met-hIgG1
Price:
0
Host Cell Line:
EXPI-CHO
Target:
MET
Species Reactivity:
Human
Application:
ELISA | ELISA Blocking | KD | FACS
Purification Method:
Affinity purified
Concentration:
>2mg/mL
Purity:
>95% by SDS-PAGE and SEC-HPLC
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Sterility:
0.2μm filtered
Formulation:
20mM sodium citrate,150mM NaCl, pH5.5
Storage:
Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.
Bio Safety Level:
1
Validation:
SDS-PAGE | SEC-HPLC | ELISA | ELISA Blocking | KD | FACS

References

1.Wang Siyun,Ma Haiqing,Yan Yan et al. cMET promotes metastasis and epithelial-mesenchymal transition in colorectal carcinoma by repressing RKIP.[J] J Cell Physiol, 2021, 236: 3963-3978. 2.Maroun,C.R.,& Rowlands,T. The Met receptor tyrosine kinase: A key player in oncogenesis and drug resistance.[J] Pharmacology and Therapeutics,2014,142(3), 316-338.
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