KC-2602

Ba/F3-NRAS-Q61H Cell Line

Home » Ba/F3-NRAS-Q61H Cell Line

Background

Specifications

Catalog Number:
KC-2602
Cell Line Name:
Ba/F3-NRAS-Q61H Cell Line
Host Cell Line:
Mouse Ba/F3 cell line
Description:
Stable Ba/F3 clone expressing exogenous NRAS bearing Q61H amino acid mutation
Quantity:
One vial of frozen cells (5×106 per vial)
Stability:
Stable in culture over a minimum of 10 passages
Application:
Drug screening and biological assays
Freeze Medium:
70% RPMI1640+20% FBS+10% DMSO
Propagation Medium:
RPMI1640+10%FBS
Selection Marker:
Puromycin
Morphology:
Mostly single, round (some polymorph) cells in suspension
Subculture:
Split saturated culture 1:10 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation:
37 °C with 5% CO2
Storage:
Liquid nitrogen immediately upon receiving
Doubling Time:
Approximately 20 hours
Mycoplasma Status:
Negative

Cell Line Generation

Characterization

Application

Cell Resuscitation

Cell Freezing

References

Please enable JavaScript in your browser to complete this form.