KB-1344

H183L072-hIgG1

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Background

Cluster of differentiation 3 (CD3) is a protein complex, historically known as the T3 complex. It consists of four distinct polypeptide chains: epsilon (¦Å), gamma (¦Ã), delta (¦Ä), and zeta (¦Æ). These chains assemble and function as three pairs of dimers (¦Å¦Ã, ¦Å¦Ä, ¦Æ¦Æ). The CD3 complex acts as a co-receptor for T cells and noncovalently associates with the T cell receptor (TCR) (Smith-Garvin et al. 2009). The CD3 protein complex is a characteristic feature of the T cell lineage, making anti-CD3 antibodies effective as T cell markers.

Specifications

Catalog Number:
KB-1344
Cell Line Name:
H183L072-hIgG1
Price:
0
Host Cell Line:
EXPI-CHO
Target:
CD3
Species Reactivity:
Human
Application:
ELISA
Purification Method:
Affinity purified
Concentration:
>2mg/mL
Purity:
>95% by SDS-PAGE and SEC-HPLC
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Sterility:
0.2¦Ìm filtered
Formulation:
20mM sodium citrate,150mM NaCl, pH5.5
Storage:
Stable for twelve months from date of receipt when stored at -20¡ãC to -80¡ãC; Stored at 2-8¡ãC for one month without detectable loss of activity.
Bio Safety Level:
1
Validation:
SDS-PAGE | SEC-HPLC | ELISA

References

1.Alarcon B et al. (1988) Assembly of the human T cell receptor CD3 complex takes place in the endoplasmic reticulum and involves intermediary complexes between the CD3¦Ã, ¦Ä, ¦Å core and single T-cell receptor a or b chains. Journal of Biological Chemistry 263, 2953-2961. 2.Clayton LK et al. (1991) CD3 eta and CD3 zeta are alternatively spliced products of a common genetic locus and are transcriptionally and/or post-translationally regulated during T-cell development. Proceedings of the National Academy of Sciences of the USA 88, 5202-5206.
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