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KC-3142

293T-CRE-Luc2-GLP1R-T2A-GIPR Cell Line

Home » 293T-CRE-Luc2-GLP1R-T2A-GIPR Cell Line

Background

Specifications

Catalog Number:
KC-3142
Cell Line Name:
293T-CRE-Luc2-GLP1R-T2A-GIPR Cell Line
Host Cell Line:
Description:
Quantity:
One vial of frozen cells (5×106 per vial)
Stability:
Stable in culture over a minimum of 10 passages
Application:
Drug screening and biological assays
Freeze Medium:
70% DMEM+20% FBS+10% DMSO
Propagation Medium:
DMEM+10%FBS+1µg/ml Puromycin+150µg/ml Hygromycin B
Selection Marker:
Puromycin
Morphology:
Epithelial
Subculture:
Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation:
37 °C with 5% CO2
Storage:
Liquid nitrogen immediately upon receiving
Doubling Time:
Approximately 30 hours
Mycoplasma Status:
Negative

Cell Line Generation

Characterization

Application

Cell Resuscitation

Cell Freezing

References

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