KB-1076-01

1452C11-mIgG2a

Background

The 145-2C11 antibody is responsive to the 20 kDa CD3εsubunit present in the T cell receptor (TCR)/CD3 complex of mice. This subunit is found on the surface of mature T cells, NKT cells, and occasionally on thymocytes. Additionally, CD3 is expressed by a majority of T cell neoplasms. The CD3 complex consists of CD3γ,δ,ε,η, andζsubunits, which assemble non-covalently with the TCR. This complex plays a crucial role in transmitting antigen recognition signals to the cytoplasm of T cells and regulates the expression of the TCR on the cell surface.

Specifications

Catalog Number:
KB-1076-01
Cell Line Name:
1452C11-mIgG2a
Price:
0
Host Cell Line:
EXPI-CHO
Target:
Mouse-CD3
Species Reactivity:
Mouse
Application:
FACS
Purification Method:
Affinity purified
Concentration:
>2mg/mL
Purity:
>95% by SDS-PAGE and SEC-HPLC
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Sterility:
0.2μm filtered
Formulation:
20mM sodium citrate,150mM NaCl, pH5.5
Storage:
Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.
Bio Safety Level:
1
Validation:
SDS-PAGE | SEC-HPLC | FACS

References

1.Hooks MA, Wade CS, Millikan WJ. Muromonab CD-3: a review of its pharmacology, pharmacokinetics, and clinical use in transplantation. Pharmacotherapy 11(1), 26–37 (1991). 2.Chatenoud L, Baudrihaye MF, Chkoff N, Kreis H, Goldstein G, Bach J-F. Restriction of the human in vivo immune response against the mouse monoclonal antibody OKT3. J. Immunol. 137(3), 830–838 (1986).
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