Mixed lymphocyte reaction (MLR) is an ex-vivo cellular immune assay that occurs between two allogeneic lymphocyte populations. The principle of MLR is that T cells from one donor will proliferate in the presence of APCs from a different donor. This is caused by the recognition of an HLA mismatch between two unrelated donors, which provokes an immune response from the T cells. MLR is often used to study the functions of immune-checkpoint antibodies through the measurement of induced IL-2 and IFN-γ expression.  

Assay Specifications:  

T cell and DC donors are pre-screened for their immune reaction. Assays are performed using a 96-well format. Each data point is tested in triplicate, and appropriate assay controls are included.

Case Study:

Turn-Around Time

2 - 3 weeks

Timeline & pricing may vary depending on custom assay specifications.